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Microbial community structure in different wastewater treatment processes characterized by single-strandconformation polymorphism (SSCP) technique

ZHAO Yangguo, WANG Aijie, REN Nanqi, ZHAO Yan

《环境科学与工程前沿（英文）》 2008年 第2卷 第1期   页码 116-121 doi: 10.1007/s11783-008-0018-8

摘要： In order to investigate microbial community structures in different wastewater treatment processes and understand the relationship between the structures and the status of processes, the microbial community diversity, variety and distribution in five wastewater treatment pro cesses were studied by a culture-independent genetic fingerprinting technique single-strand conformation polymorphism (SSCP). The five processes included denitrifying and phosphate-removal system (diminished N), Chinese traditional medicine wastewater treatment system (P), beer wastewater treatment system (W), fermentative biohydrogen-producing system (H), and sulfate-reduction system (S). The results indicated that the microbial community profiles in the wastewater bioreactors with the uniform status were very similar. The diversity of microbial populations was correlated with the complexity of organic contaminants in wastewater. Chinese traditional medicine wastewater contained more complex organic components; hence, the population diversity was higher than that of simple nutrient bioreactors fed with molasses wastewater. Compared with the strain bands in a simulated community, the relative proportion of some functional microbial populations in bioreactors was not dominant. Fermentative biohydrogen producer in the better condition bioreactor had only a 5% band density, and the sp. in the sulfate-reducing bioreactor had less than 1.5% band density. The SSCP profiles could identify the difference in microbial community structures in wastewater treatment processes, monitor some of the functional microbes in these processes, and consequently provide useful guidance for improving their efficiency.

关键词： technique single-strand     proportion     bioreactor     community diversity     fingerprinting technique    

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Single incision laparoscopic cholecystectomy using the one-incision three-trocar technique with all straight

Hongyi Cui

《医学前沿（英文）》 2011年 第5卷 第3期   页码 283-287 doi: 10.1007/s11684-011-0151-9

摘要： Single incision laparoscopic surgery (SILS) is a novel minimally invasive surgical technique that is gaining popularity around the world. One of the most commonly performed procedures is single incision laparoscopic cholecystectomy (SILC). Most reported techniques utilize special purpose-made access port and articulating instruments, rendering the procedure costly and difficult to learn. This article provides a stepwise description of SILC technique using all straight instruments without the need for a special port. It aims to shorten the learning curve for surgeons wishing to adopt a safe and cost-effective SILC technique to their practice.

关键词： laparoscopic cholecystectomy     single incision laparoscopic surgery    

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Secondary transfer length and residual prestress of fractured strand in post-tensioned concrete beams

Lizhao DAI; Wengang XU; Lei WANG; Shanchang YI; Wen CHEN

《结构与土木工程前沿（英文）》 2022年 第16卷 第3期   页码 388-400 doi: 10.1007/s11709-022-0809-1

摘要： An experimental study is performed on five post-tensioned concrete beams to explore the effects of different fracture positions on secondary transfer length and residual prestress of fractured strand. A numerical model is developed and used to predict the secondary transfer length and residual prestress of fractured strand in post-tensioned concrete beams. The model change interaction, which can deactivate and reactivate the elements for simulating the removal and reproduction of parts of the model, is used to reproduce the secondary anchorage of fractured strand. The numerical model is verified by experimental results. Results shows that the fractured strand can be re-anchored in concrete through the secondary anchorage, and the secondary transfer length of fractured strand with the diameter of 15.2 mm is 1133 mm. The residual prestress of fractured strand increases gradually in the secondary transfer length, and tends to be a constant beyond it. When the fractured strand is fully anchored in concrete, a minor prestress loss will appear, and the average prestress loss is 2.28% in the present study.

关键词： post-tensioned concrete beams     strand fracture     secondary transfer length     residual prestress    

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悬索桥主缆单元索股水平收放索技术应用智能放索装置研制

沈旺,俞建群,顾庆华,毛优达

《中国工程科学》 2010年 第12卷 第7期   页码 4-7

摘要：

介绍了悬索桥主缆单元索股放索时，针对水平收放索技术的一种被动式智能放索装置的设计原理、组装和试验，以及在使用此智能放索装置后，主缆单元索股放索质量的提高。

关键词： 悬索桥     主缆单元索股     智能     放索装置    

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Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

《农业科学与工程前沿（英文）》 2014年 第1卷 第3期   页码 185-190 doi: 10.15302/J-FASE-2014035

摘要： DNA damage is one of the most common threats to meiotic cells. It has the potential to induce infertility and genetic abnormalities that may be passed to the embryo. Here, we reviewed exogenous factors which could induce DNA damage. Specially, we addressed the different effects of DNA damage on mouse oocytes and embryonic development. Complex DNA damage, double-strand breaks, represents a more difficult repair process and involves various repair pathways. Understanding the mechanisms involved in DNA damage responses may improve therapeutic strategies for ovarian cancer and fertility preservation.

关键词： DNA damage     double-strand breaks (DSBs)     oocyte     embryo    

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超长大规格高强度悬索桥主缆索股编制装备技术研究

段素敬,张海良,朱金林,沈旺,毛优达

《中国工程科学》 2010年 第12卷 第7期   页码 18-21

摘要：

阐述了超长大规格高强度悬索主缆索股编制装备，为悬索主缆单元索股的工厂化制作提供可靠的工业装备，满足了大规模生产的需要。

关键词： 超长大规格高强度     悬索     主缆索股     编制装备    

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Correlativity study between expression of DNA double-strand break repair protein and radiosensitivity

Liang ZHUANG, Shiying YU, Xiaoyuan HUANG, Yang CAO, Huihua XIONG

《医学前沿（英文）》 2009年 第3卷 第1期   页码 26-29 doi: 10.1007/s11684-009-0008-7

摘要： DNA double-strand break (DSB) is generally regarded as the most lethal of all DNA lesions after radiation. Ku80, DNA-PK catalytic subunit (DNA-PKcs) and ataxia telangiectasia mutated (ATM) proteins are major DSB repair proteins. In this study, survival fraction at 2Gy (SF2) values of eight human tumor cell lines (including four human cervical carcinoma cell lines HeLa, SiHa, C33A, Caski, three human breast carcinoma cell lines MCF-7, MDA-MB-231, MDA-MB-453, and one human lung carcinoma cell line A549) were acquired by clone formation assay, and western blot was applied to detect the expressions of Ku80, DNA-PKcs and ATM protein. The correlativity of protein expression with SF2 value was analyzed by Pearson linear correlation analysis. We found that the expression of same protein in different cell lines and the expression of three proteins in the same cell line had a significant difference. The SF2 values were also different in eight tumor cell lines and there was a positive correlativity between the expression of DNA-PKcs and SF2 ( ＝0.723, = 0.043), but Ku80 and ATM expression had no correlation with SF2 ( >0.05). These findings suggest that the expression level of DNA-PKcs protein can be an indicator for predicting the radiosensitivity of tumor cells.

关键词： Ku80     DNA-PK(cs)-binding protein     human     ataxia telangiectasia mutated protein     tumor cell lines     radiosensitivity    

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General modeling and optimization technique for real-world earth observation satellite scheduling

《工程管理前沿（英文）》   页码 695-709 doi: 10.1007/s42524-023-0263-3

摘要： Over the last two decades, many modeling and optimization techniques have been developed for earth observation satellite (EOS) scheduling problems, but few of them show good generality to be engineered in real-world applications. This study proposes a general modeling and optimization technique for common and real-world EOS scheduling cases; it includes a decoupled framework, a general modeling method, and an easy-to-use algorithm library. In this technique, a framework that decouples the modeling, constraints, and optimization of EOS scheduling problems is built. With this framework, the EOS scheduling problems are appropriately modeled in a general manner, where the executable opportunity, another format of the well-known visible time window per EOS operation, is viewed as a selectable resource to be optimized. On this basis, 10 types of optimization algorithms, such as Tabu search and genetic algorithm, and a parallel competitive memetic algorithm, are developed. For simplified EOS scheduling problems, the proposed technique shows better performance in applicability and effectiveness than the state-of-the-art algorithms. In addition, a complicatedly constrained real-world benchmark exampled by a four-EOS Chinese commercial constellation is provided, and the technique is qualified and outperforms the in-use scheduling system by more than 50%.

关键词： earth observation satellite     scheduling     general technique     optimization algorithm     commercial constellation     real-world     benchmark    

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Mechanisms and impacts of chromosomal translocations in cancers

null

《医学前沿（英文）》 2012年 第6卷 第3期   页码 263-274 doi: 10.1007/s11684-012-0215-5

摘要：

Chromosomal aberrations have been associated with cancer development since their discovery more than a hundred years ago. Chromosomal translocations, a type of particular structural changes involving heterologous chromosomes, have made a critical impact on diagnosis, prognosis and treatment of cancers. For example, the discovery of translocation between chromosomes 9 and 22 and the subsequent success of targeting the fusion product BCR-ABL transformed the therapy for chronic myelogenous leukemia. In the past few decades, tremendous progress has been achieved towards elucidating the mechanism causing chromosomal translocations. This review focuses on the basic mechanisms underlying the generation of chromosomal translocations. In particular, the contribution of frequency of DNA double strand breaks and spatial proximity of translocating loci is discussed.

关键词： DNA double strand breaks     chromosomal translocations     genomic instability     spatial proximity     carcinogenesis    

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超长大规格主缆单元索股成圈束股运输技术研究

陆剑峰,顾庆华,张海良,沈旺,周颂

《中国工程科学》 2010年 第12卷 第7期   页码 13-17

摘要：

结合主跨1 650 m西堠门大桥主缆索股的运输技术，以及规格Ø5.1×169丝×4 250 m重120 t 的主缆单元索股的实例，介绍了超长大规格悬索主缆成圈束股的运输技术。

关键词： 超长     大规格     单元索股     成圈束股     运输    

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Crystallographic orientation effect on cutting-based single atomic layer removal

Wenkun XIE, Fengzhou FANG

《机械工程前沿（英文）》 2020年 第15卷 第4期   页码 631-644 doi: 10.1007/s11465-020-0599-x

摘要： The ever-increasing requirements for the scalable manufacturing of atomic-scale devices emphasize the significance of developing atomic-scale manufacturing technology. The mechanism of a single atomic layer removal in cutting is the key basic theoretical foundation for atomic-scale mechanical cutting. Material anisotropy is among the key decisive factors that could not be neglected in cutting at such a scale. In the present study, the crystallographic orientation effect on the cutting-based single atomic layer removal of monocrystalline copper is investigated by molecular dynamics simulation. When undeformed chip thickness is in the atomic scale, two kinds of single atomic layer removal mechanisms exist in cutting-based single atomic layer removal, namely, dislocation motion and extrusion, due to the differing atomic structures on different crystallographic planes. On close-packed crystallographic plane, the material removal is dominated by the shear stress-driven dislocation motion, whereas on non-close packed crystallographic planes, extrusion-dominated material removal dominates. To obtain an atomic, defect-free processed surface, the cutting needs to be conducted on the close-packed crystallographic planes of monocrystalline copper.

关键词： ACSM     single atomic layer removal mechanism     crystallographic orientation effect     mechanical cutting     Manufacturing III    

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Design and analysis of partially decoupled translational parallel mechanisms with single-loop structures

《机械工程前沿（英文）》 2022年 第17卷 第3期 doi: 10.1007/s11465-022-0695-1

摘要： This study presents a family of novel translational parallel mechanisms (TPMs) with single-loop topological structures. The proposed mechanism consists of only revolute and prismatic joints. The novel TPMs are simpler in structure and have fewer joints and components than the well-known Delta Robot. Four types of 2-degree of freedom driving systems are applied to different limb structures to avoid the moving actuator that causes the problem of increased moving mass. Four sample TPMs are constructed using the synthesized limbs, and one of them is investigated in terms of kinematic performance. First, a position analysis is performed and validated through numerical simulation to reveal the characteristics of partially decoupled motion, which improves the controllability of TPM. Second, singular configurations are identified, and the resulting singularity curve is obtained. Lastly, the workspace of TPM is analyzed, and the relationship between the singular configurations and the reachable workspace is explored. The workspace of the 3-CRR (C denotes the cylindrical joint and R denotes the revolute joint) translational mechanism is also presented to prove that the proposed TPM has a fairly large workspace.

关键词： translational parallel mechanism     single loop     multiple driving system     workspace analysis     singularity    

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Moving policy and regulation forward for single-use plastic alternatives

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-021-1423-5

摘要： Single-use plastics are often used once or cannot be reused for extended periods. They are widely consumed with the rapid development of social economy. The waste generated by single-use plastics threatens ecosystem health by entering the environment and ultimately restricts sustainable human development. The innovation of sustainable and environmentally friendly single-use plastic alternative materials and the joint participation of governments, enterprises and the public are promising technologies and management approaches that can solve the problem of single-use plastics wastes. The development of single-use plastic alternative products can be promoted fundamentally only by improving relevant legislation and standards, providing differentiated industrial policies, encouraging scientific and technological innovation and expanding public participation.

关键词： Single-use plastic alternatives     Policy     Regulation     Sustainable development    

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构建我国农业信息化技术支持体系的探讨

郭书普,张立平,沈基长,董伟

《中国工程科学》 2005年 第7卷 第9期   页码 89-94

摘要：

阐述了农业信息化的基本特征，介绍了农业信息化技术支持体系的发展现状，分析了存在的问题，指出现阶段加快农业信息技术体系建设应当重点抓好农业信息贮藏技术的研究、农业信息应用技术的开发、农业信息传播网络的建设。

关键词： 农业信息化     技术支持体系     信息贮藏技术     信息应用技术     信息传播网络    

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Damage propagation and strength prediction of a single-lap interference-fit laminate structure

Peng ZOU, Xiangming CHEN, Hao CHEN, Guanhua XU

《机械工程前沿（英文）》 2020年 第15卷 第4期   页码 558-570 doi: 10.1007/s11465-020-0591-5

摘要： Experimental and finite element research was conducted on the bolted interference fit of a single-lap laminated structure to reveal the damage propagation mechanism and strength change law. A typical single-lap statically loading experiment was performed, and a finite element damage prediction model was built based on intralaminar progress damage theory. The model was programmed with a user subroutine and an interlaminar cohesive zone method. The deformation and damage propagation of the specimen were analyzed, and the failure mechanism of intralaminar and interlaminar damage during loading was discussed. The effect of secondary bending moment on load translation and damage distribution was revealed. The experimental and simulated load–displacement curves were compared to validate the developed model’s reliability, and the ultimate bearing strengths under different fit percentages were predicted. An optimal percentage was also recommended.

关键词： single-lap     interference fit     secondary bending moment     damage mechanism     bearing strength    

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标题 作者 时间 类型 操作

Microbial community structure in different wastewater treatment processes characterized by single-strandconformation polymorphism (SSCP) technique

ZHAO Yangguo, WANG Aijie, REN Nanqi, ZHAO Yan

期刊论文

Single incision laparoscopic cholecystectomy using the one-incision three-trocar technique with all straight

Hongyi Cui

期刊论文

Secondary transfer length and residual prestress of fractured strand in post-tensioned concrete beams

Lizhao DAI; Wengang XU; Lei WANG; Shanchang YI; Wen CHEN

期刊论文

悬索桥主缆单元索股水平收放索技术应用智能放索装置研制

沈旺,俞建群,顾庆华,毛优达

期刊论文

Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

期刊论文

超长大规格高强度悬索桥主缆索股编制装备技术研究

段素敬,张海良,朱金林,沈旺,毛优达

期刊论文

Correlativity study between expression of DNA double-strand break repair protein and radiosensitivity

Liang ZHUANG, Shiying YU, Xiaoyuan HUANG, Yang CAO, Huihua XIONG

期刊论文

General modeling and optimization technique for real-world earth observation satellite scheduling

期刊论文

Mechanisms and impacts of chromosomal translocations in cancers

null

期刊论文

超长大规格主缆单元索股成圈束股运输技术研究

陆剑峰,顾庆华,张海良,沈旺,周颂

期刊论文

Crystallographic orientation effect on cutting-based single atomic layer removal

Wenkun XIE, Fengzhou FANG

期刊论文

Design and analysis of partially decoupled translational parallel mechanisms with single-loop structures

期刊论文

Moving policy and regulation forward for single-use plastic alternatives

期刊论文

构建我国农业信息化技术支持体系的探讨

郭书普,张立平,沈基长,董伟

期刊论文

Damage propagation and strength prediction of a single-lap interference-fit laminate structure

Peng ZOU, Xiangming CHEN, Hao CHEN, Guanhua XU

期刊论文